REVIEW The restriction site mutation (RSM) method: clinical applications

Enhanced restriction site mutation (RSM) analysis of 1,2-dimethylhydrazine induced mutations, using endogenous p53 intron sequences.

The restriction site mutation (RSM) assay was used to study the mutational sensitivities of three target regions of the murine p53 gene. The non-coding intron 6 target region was compared with the coding regions exon 4 and exon 5 with respect to their relative sensitivity to the induction of mutations by 1,2-dimethylhydrazine (DMH). Our results demonstrated that the majority of induced mutation...

Universal restriction site-free cloning method using chimeric primers.

A universal restriction site-free cloning method has been developed to precisely insert a DNA fragment into a vector at any desired location without altering any nucleotide(s) in either the DNA fragment or the vector. The technique employs two pairs of chimeric primers, each containing a ribonucleotide. One pair of primers is used to amplify a target DNA fragment and another is used to prepare ...

Peer Review of the Regional Simulation Model ( RSM )

Methodological Principles and Applications of the Delphi Method: A Narrative Review

Background and Objectives: The appearance of complex issues with insufficient information has resulted in the consensus or unanimity spread. The consensus methods include the nominal group and the Delphi method.The Delphi method is a systematic process to predict and help making decision through survey rounds and information gathering and finally the group consensus. This method has been design...

Mutation detection, interpretation, and applications in the clinical laboratory setting.

Mutation detection plays an increasingly significant role in clinical diagnostic testing, posing formidable challenges for laboratories. The expanding indications for clinical molecular testing and the nuances of interpreting test results are discussed. Methods for screening mutation detection platforms and monitoring assay reliability are presented, and results of platform comparisons are desc...